HDL Mimetics Enhances Mitochondrial Function via Stimulation of PGC1-α

Register or Login to View PDF Permissions
Permissions× For commercial reprint enquiries please contact Springer Healthcare:

For permissions and non-commercial reprint enquiries, please visit to start a request.

For author reprints, please email
Information image
Average (ratings)
No ratings
Your rating
Open Access:

This work is open access under the CC-BY-NC 4.0 License which allows users to copy, redistribute and make derivative works for non-commercial purposes, provided the original work is cited correctly.

Objective: Various large clinical trials have shown that high-density lipoprotein (HDL) has pleiotropic effects for anti-atherosclerosis, and an enhanced HDL by CETP inhibitor improves glucose metabolism. In addition, HDL and apolipoprotein A-I (ApoA- I), the major protein of HDL had been demonstrated to enhance mitochondrial function in skeletal muscle in vitro.

Materials and methods: One of the HDL mimetics, Fukuoka University ApoA-I Mimetic Peptide (FAMP) was developed as a low-amino acid residues peptide preserving human ApoA-I activity without phospholipids and has been reported to enhance HDL functions. C57BL6J mice were intraperitoneally administered 50 mg/kg/day of FAMP or saline for 4 weeks. After 4 weeks, plasma samples were collected, and HDL was extracted by ApoB-depleted method. The mitochondrial functions were evaluated with the extracellular flux analyser in C2C12 mouse myoblast cells ex vivo.

Results: HDL induced oxygen consumption rate changes that was the significant elevation of basal respiration, maximal respiration, ATP production and spare respiratory capacity (+35%, +54%, +35%, +68%, respectively). Moreover, HDL from mice treated with FAMP has further increasing maximal respiration and spare respiratory capacity, significantly. In addition, mice HDL from 4 weeks treatment with FAMP significantly increased PPARg-coactivator 1-α mRNA expression (HDL, 9.6 ± 2.0; HDL treated with FAMP, 12.2 ± 3.6; p<0.01).

Conclusion: Our results reveal that treatment with HDL mimetics improves mitochondrial function in skeletal muscle cells through stimulation of PGC1-α expressions. These findings may suggest that HDL prevents cardiovascular disease by enhancement of skeletal muscle functions.